|
Finding MPLA probes.
This assignment was about finding MPLA probes. I had to make a couple of
changes to an existing program to implement this. See
this paper for the complete description of the
assignment.
- Get release 0.9 of Primer3.
- Get this patch.
- Patch the source code by doing:
- cd primer3_0_9_test
- patch -p1 <
../primer3_0_9_test_p/primer3_0_9_test_p.patch
- Recompile the code.
Primer3 will now recognize two more input tags:
- PRIMER_CONTACT (Default =0, set to =1 to find two primers with no space
between them).
- PRIMER_SAME_STRAND (Default =0, set to =1 to find primers on the same
strand).
The patch archive also contains a patch for this perl
script. This script iteratively calls the Primer3 executable.
Look here
for more information on Primer3.
|
